Publication： Proceedings Of The National Academy Of Sciences

Date of Publication: October 1, 2008

Authors： Eugene Antipova, Art E. Cho, K. Dane Wittrupa, and Alexander M. Klibanova

DOI: 10.1073_pnas.0809851105

A highly efficient selection method for enhanced enzyme enantioselectivity based on yeast surface display and fluorescence-activated cell sorting (FACS) is developed and validated. Its application to horseradish peroxidase has resulted in enzyme variants up to 2 orders of magnitude selective toward either substrate enantiomer at will. These marked improvements in enantioselectivity are demonstrated for the surface-bound and soluble enzymes and rationalized by computational docking studies.